Imikhiqizo
I-Trimethylol aminomethane
Sebenzisa
① I-fosfomycin emaphakathi, ingasetshenziswa futhi njengesisheshisi se-vulcanization, izimonyo (ukhilimu, amafutha okugcoba), uwoyela wamaminerali, i-emulsifier kapharafini, isigcinalwazi sebhayoloji.
② Isetshenziswa njenge-asidi emunca igesi, ukulungiswa kwebhafa, i-surfactant, i-emulsifier ne-accelerator.Ibuye isetshenziswe kuma-organic synthes.
Indlela Yokukhiqiza
Chaza i-Tris ngenani elincane lamanzi abilayo aphindwe kabili (300-500ml), engeza i-HCl, lungisa i-pH ibe ngu-7.6 nge-HCl (1N) noma i-NaOH (1N), futhi ekugcineni wengeze amanzi abilayo kabili ku-1000ml.Lolu ketshezi luwuketshezi olugcinayo, olugcinwe esiqandisini ngo-4℃.
Qaphela: Inani elingu-PH le-Tris-Hcl liyahlukahluka ngokwezinga lokushisa, ngakho-ke kufanele likalwe kuzinga lokushisa legumbi, ukuze imiphumela elinganisiwe ithembeke kakhulu.
Isici sokubhafa
I-Tris isisekelo esibuthakathaka esine-pKa engu-8.1 ekamelweni lokushisa (25℃).Ngokombono we-buffering, uhla olusebenzayo lokubhafa lwe-Tris buffers luphakathi kwe-pH7.0 kanye no-9.2.
I-pH yesisombululo esinamanzi sesisekelo se-Tris icishe ibe ngu-10.5, futhi isigcinalwazi senani le-pH singatholwa ngokwengeza i-hydrochloric acid ukuze kulungiswe inani le-pH kunani elifunekayo.Kodwa-ke, kufanele kuqashelwe ithonya lokushisa ku-pKa ye-Tris.Idigri, inani le-PH lehle ngo-0.03.
I-1M Tris-HCl 6.8 kanye ne-1.5M Tris-HCl 8.8 ama-reagents asetshenziswa kakhulu e-SDS-PAGE.
Nakuba i-TAE, i-TBE namanye ama-reagents ahlanganiswe asuka ku-Tris kuyizinto ezisetshenziswa kakhulu ukwenza i-DNA electrophoresis, i-TE (pH8.0) isetshenziswa kakhulu ekuhlakazweni kwe-DNA.(I-TE ithi Tris kanye ne-EDTA.)
Amabhafa e-Tris awasetshenziswa kabanzi nje kuphela njengezincibilikisi zama-nucleic acid namaprotheni, kodwa futhi anokusetshenziswa okuningi okubalulekile.I-Tris isetshenziselwa ukukhula kwekristalu yamaprotheni ngaphansi kwezimo ezihlukene ze-pH.Amandla e-ionic aphansi webhafa ye-Tris angasetshenziselwa ukwakheka kwemicu emaphakathi ye-lamin kuma-C. elegans.I-Tris futhi ingenye yezingxenye eziyinhloko zama-protein electrophoresis buffers.Ngaphezu kwalokho, i-Tris iyingxenye emaphakathi ekulungiseni ama-surfactants, ama-vulcanization accelerators, nezinye izidakamizwa.I-Tris iphinde isetshenziswe njengezinga le-titration.
Ngesimo sezithombe nombhalo, kungabandakanya ulwazi lomkhiqizo, izinsizakalo kanye nezinzuzo.Ingadlulela ngale komkhiqizo ukuze ingeze okuqukethwe okuthile (okukhathaza ikhasimende, ukukhathazeka, njll.), ukuvikelwa kwemvelo, amasampula amahhala, njll.
1. Amasampula ahlolwa mahhala ukuze kuqinisekiswe ukusetshenziswa okuvamile kwamakhasimende.
2. Ngokwesidingo samakhasimende ahlukene, amancane kuya ku-100g, amakhulu kuya kumathani emiphongolo, angahlangabezana nezidingo zokupakisha.
3. Imigomo yokukhokha eguquguqukayo, ukudluliswa kwefoni noma ukwamukela (hlangabezana nezimfuneko zokwamukela)
4. Ukuthutha okusheshayo, ngosuku olufanayo noma ngakusasa kungalethwa, yonke inqubo yokulandelela ulwazi lwezokuthutha, ukuqinisekisa ukusetshenziswa okufika ngesikhathi kwamakhasimende.
5. Isevisi enhle kakhulu yangemva kokuthengisa, enesineke futhi ecophelelayo ukuxazulula zonke izinhlobo zezinkinga ezingase zenzeke, njengezinkinga zekhwalithi, sebenzisana ngokugcwele namakhasimende ukubhekana nawo ngenkuthalo, ungakubalekeli umthwalo wemfanelo kanye nokuphendula okungenzi lutho.
6. Ithimba elihle kakhulu, ukusebenza kahle okusebenzayo nolwazi lochwepheshe kwenza amakhasimende angagcini nje ngokuqiniseka ngemikhiqizo yethu, kodwa futhi abe nomuzwa wokuthi ithimba lethu lithembekile.
7. Ilungelo lokuthekelisa elizimele lokwakha imakethe yamazwe ngamazwe kanye nomthelela womkhiqizo womhlaba wonke.
8. Abaqotho, abasekelwe encwadini, iminyaka engu-20 yomlando wenkampani kanye nedumela elihle, vumela amakhasimende ukuthi akhululeke, azuze ngokufanayo futhi awine, ukubambisana okuthembekile.
